Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2.

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Title

Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2.

Subject

Description

Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2-3 targets for detection.

Date Last Updated (Year-Month-Day)

2020-04-15

Citation

Jesse, J. Waggoner, Stittleburg Victoria, Pond Renee, Saklawi Youssef, K. Sahoo Malaya, Babiker Ahmed, Hussaini Laila, S. Kraft Colleen, A. Pinsky Benjamin, J. Anderson Evan, and Rouphael Nadine. 2020. "Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2." Emerging Infectious Disease journal 26 (7).

Abstract

Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2-3 targets for detection. We developed a triplex, real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance compared with singleplex assays. This protocol could streamline detection and decrease reagent use during current high SARS-CoV-2 testing demands.

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