Viable influenza A virus in airborne particles from human coughs

Item Type:

Publication

Title

Viable influenza A virus in airborne particles from human coughs

Subject

Description

Patients with influenza release aerosol particles containing the virus into their environment.

Date Last Updated (Year-Month-Day)

2015

Citation

Lindsley, William G., John D. Noti, Francoise M. Blachere, Robert E. Thewlis, Stephen B. Martin, Sreekumar Othumpangat, Bahar Noorbakhsh, William T. Goldsmith, Abhishek Vishnu, Jan E. Palmer, Karen E. Clark, and Donald H. Beezhold. 2015. "Viable Influenza A Virus in Airborne Particles from Human Coughs." Journal of Occupational and Environmental Hygiene 12 (2):107-13.

Abstract

Abstract

Patients with influenza release aerosol particles containing the virus into their environment. However, the importance of airborne transmission in the spread of influenza is unclear, in part because of a lack of information about the infectivity of the airborne virus. The purpose of this study was to determine the amount of viable influenza A virus that was expelled by patients in aerosol particles while coughing. Sixty-four symptomatic adult volunteer outpatients were asked to cough 6 times into a cough aerosol collection system. Seventeen of these participants tested positive for influenza A virus by viral plaque assay (VPA) with confirmation by viral replication assay (VRA). Viable influenza A virus was detected in the cough aerosol particles from 7 of these 17 test subjects (41%). Viable influenza A virus was found in the smallest particle size fraction (0.3 μm to 8 μm), with a mean of 142 plaque-forming units (SD 215) expelled during the 6 coughs in particles of this size. These results suggest that a significant proportion of patients with influenza A release small airborne particles containing viable virus into the environment. Although the amounts of influenza A detected in cough aerosol particles during our experiments were relatively low, larger quantities could be expelled by influenza patients during a pandemic when illnesses would be more severe. Our findings support the idea that airborne infectious particles could play an important role in the spread of influenza.

KEYWORDS:

aerosols; air microbiology; airborne transmission; cough; infectious disease transmission; influenza

Accessibility

Free author manuscript on PubMed Central, published version through Taylor and Francis