NETEC Resource Library

Quantification of Influenza Virus RNA in Aerosols in Patient Rooms

Contenu

Click for External Resource*


Click to read full article*


*The link above may share a zip file (.zip) hosted on repository.netecweb.org. Zip files will download automatically.
*All other links are external and will open in a new window. If you click an external link, you are leaving the NETEC site, and we do not maintain, review, or endorse these materials. See our terms of use.


Files for Download

journal.pone.0148669.PDFjournal.pone.0148669.PDF

Item Type

Publication

Terms of Use

By accessing these materials you are agreeing to our terms of use, which may be found here: Terms of Use.

Voir les documents

Was this resource helpful?


Titre

Quantification of Influenza Virus RNA in Aerosols in Patient Rooms

Sujet

Description

The potential for human influenza viruses to spread through fine particle aerosols remains controversial.

Date

2016-02-05

Citer ce document

Leung, Nancy H. L., Jie Zhou, Daniel K. W. Chu, Han Yu, William G. Lindsley, Donald H. Beezhold, Hui-Ling Yen, Yuguo Li, Wing-Hong Seto, Joseph S. M. Peiris, and Benjamin J. Cowling. 2016. "Quantification of Influenza Virus RNA in Aerosols in Patient Rooms." PLoS One 11 (2):e0148669-e.

Résumé

Abstract

BACKGROUND:

The potential for human influenza viruses to spread through fine particle aerosols remains controversial. The objective of our study was to determine whether influenza viruses could be detected in fine particles in hospital rooms.

METHODS AND FINDINGS:

We sampled the air in 2-bed patient isolation rooms for four hours, placing cyclone samplers at heights of 1.5m and 1.0m. We collected ten air samples each in the presence of at least one patient with confirmed influenza A virus infection, and tested the samples by reverse transcription polymerase chain reaction. We recovered influenza A virus RNA from 5/10 collections (50%); 4/5 were from particles>4 μm, 1/5 from 1-4 μm, and none in particles<1 μm.

CONCLUSIONS:

Detection of influenza virus RNA in aerosols at low concentrations in patient rooms suggests that healthcare workers and visitors might have frequent exposure to airborne influenza virus in proximity to infected patients. A limitation of our study was the small sample size. Further studies should be done to quantify the concentration of viable influenza virus in healthcare settings, and factors affecting the detection of influenza viruses in fine particles in the air.

Accessibilité

Free online (open access)

Collection

Related Resource Topic Exhibits