Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2.
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Título
Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2.
Materia
Descripción
Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2-3 targets for detection.
Fecha
2020-04-15
Tipo
Citación
Jesse, J. Waggoner, Stittleburg Victoria, Pond Renee, Saklawi Youssef, K. Sahoo Malaya, Babiker Ahmed, Hussaini Laila, S. Kraft Colleen, A. Pinsky Benjamin, J. Anderson Evan, and Rouphael Nadine. 2020. "Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2." Emerging Infectious Disease journal 26 (7).
Resumen
Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2-3 targets for detection. We developed a triplex, real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance compared with singleplex assays. This protocol could streamline detection and decrease reagent use during current high SARS-CoV-2 testing demands.
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