Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2.
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Titre
Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2.
Sujet
Description
Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2-3 targets for detection.
Date
2020-04-15
Type
Citer ce document
Jesse, J. Waggoner, Stittleburg Victoria, Pond Renee, Saklawi Youssef, K. Sahoo Malaya, Babiker Ahmed, Hussaini Laila, S. Kraft Colleen, A. Pinsky Benjamin, J. Anderson Evan, and Rouphael Nadine. 2020. "Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2." Emerging Infectious Disease journal 26 (7).
Résumé
Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2-3 targets for detection. We developed a triplex, real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance compared with singleplex assays. This protocol could streamline detection and decrease reagent use during current high SARS-CoV-2 testing demands.
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